Extended Data Fig. 4: Purification of active, methylated PP2A:B55 from human cells.
From: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment
a. Schematic of the production of PP2A:B55 and PP2A:B55-substrate complexes for structural and biophysical studies. b. Size exclusion chromatography of PP2A:B55, demonstrating the PP2A-B55 complex is stable and elutes as a single peak (Peak 1) with excess B55 eluting separately (peak 2), as shown in stain-free SDS-PAGE (representative of >20 experiments). c. SDS-PAGE of PP2A:B55-p107 and PP2A:B55-Eya3 samples (top; n = 2: cryo-EM samples prepared twice for p107 and EYA3), including protein and detergent concentrations (bottom), used for cryo-EM.
