Extended Data Fig. 6: Cryo-EM image processing workflow and maps for PP2A:B55-Eya3.
From: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment
a. Micrograph showing individual particles. b. Particle counts and reconstruction resolution are given at key junctions of the process (all resolutions reported are calculated by the gold standard half-maps FSC = 0.143 criterion). c. Reference-free 2D class averages generated from the 107,919 particles used in the final refinement. d. The global resolution estimate from the masked Fourier Shell Correlation curve is 2.70 Å at FSC of 0.143. e. Angular distribution calculated in cryoSPARC for the 107,919 particles included in this final reconstruction. f. Cryo-EM map colored by local resolution. g. Density maps and models for different regions of the PP2A:B55-EYA3 complex.
