Extended Data Fig. 7: PP2A:B55 preferentially dephosphorylates p107 CDK2/Cyclin A2 phosphosite pS640, full spectra.
From: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment
a. 2D [1H,15N] HSQC spectrum of 15N-labeled unphosphorylated p107 (black) overlaid with that of CDK2/Cyclin A2 phosphorylated 15N-labeled p107 (purple); pS615, pS640 and shifted peaks labeled. b-h. 2D [1H,15N] HSQC spectra of 15N-labeled unphosphorylated p107 (black) overlaid with that of CDK2/Cyclin A2 phosphorylated 15N-labeled p107 incubated with PP2A:B55 (red) for the following timepoints in minutes: 45 (b), 73 (c), 129 (d), 185 (e), 213 (f), 409 (g) and 1277 (h).
