Extended Data Fig. 2: p107 binds PP2A:B55.
From: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment
a. Pull-down assay schemes, with input and GFP-IP steps indicated. b. Expi293F cell lysates expressing GFP-B55, 3xFLAG-PP2Ac and 6xHis-PP2Aa were incubated with or without purified p107 (residues 612-687), immunopurified using GFP-trap beads (EGFP nanobodies coupled to agarose resin) and isolated proteins detected by immunoblot. Results representative of 3 independent experiments. c. Chemical Shift Index (CSI) (left) and Secondary-structure propensity (SSP) (right) data for p107 plotted vs. residue numbers. (SSP > 0, α helix; SSP < 0, β strand). Cα and Cβ chemical shifts were used to create the CSI and SSP plots (RefDB database)1. Preferred secondary structure indicated above SSP data.
