Table 1 Cryo-EM data collection, refinement and validation statistics
From: Cryo-EM structures of PP2A:B55 with p107 and Eya3 define substrate recruitment
|  | PP2A:B55–p107 | PP2A:B55–Eya3 |
Data collection and processing | ||
Magnification | 105,000x | 165,000x |
Voltage (keV) | 300 | 300 |
Exposure dose | 39.6 | 51.04 |
Defocus range (μm) | −0.55 to −2.6 | −0.6 to −2.0 |
Pixel size (Ã…) | 0.827 | 0.73 |
Symmetry imposed | C1 | C1 |
Initial particle images (no.) | 2,800,783 | 874,057 |
Final particle images (no.) | 195,950 | 107,919 |
Map resolution (Ã…) | 2.6 | 2.7 |
 FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 1.8–24.9 | 1.7–18.8 |
Refinement | ||
Initial model used (PDB code) | ||
Model Composition | ||
 Chains | 4 | 4 |
 Atoms | 10,736 | 10,659 |
 Residues | 1,344 | 1,333 |
 Ligands | Zn: 1, Fe: 1 | Zn: 1, Fe: 1 |
Root-mean-square deviations | ||
 Bond lengths (Å) | 0.003 | 0.004 |
 Bond angles (°) | 0.475 | 0.538 |
B factors (Ã…2) min/max/mean | ||
 Protein | 85.0/190.05/128.9 | 43.4/140.4/81.2 |
 Ligand | 160.4/164.9/162.7 | 112.3/113.2/112.7 |
Map-model fit (Ã…) | 2.8 | 2.7 |
 FSC | 0.5 | 0.5 |
Validation | ||
 MolProbity score | 1.26 | 1.32 |
 Clashscore | 3.89 | 4.01 |
 Rotamer outliers (%) | 0.00 | 0.00 |
Ramachandran plot (%) | ||
 Outliers | 0.00 | 0.08 |
 Allowed | 2.4 | 2.7 |
 Favored | 97.6 | 97.3 |
Rotamer outliers (%) | 0.00 | 0.00 |
Cβ outliers (%) | 0.00 | 0.00 |
