Extended Data Fig. 3: Gpd1 deletion impairing Imh1 localization is not via affecting Drs2 activity.
From: Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1
(a) gpd1∆ cells do not show sensitivity to cinnamycin. Cells were cultured to mid-log phase and treated with or without 30 μM cinnamycin for 6 h at 30 °C. The growth percentage every 1 h was determined by dividing the optical density of the treated strain obtained at 600 nm by that of the untreated control. (b) Glycerol fails to restore mislocalized Imh1 in drs2∆ cells. mCH-Imh1 was expressed in WT and drs2∆ cells. Cells were grown to mid-log phase in YPD medium and then transferred to YPG medium (YP medium containing 0.1 M glycerol) for 10 min. The number of mCH-Imh1 puncta was determined using ImageJ Fiji software (N = 3, n = 50). Data are presented as the mean ± SD of three independent experiments. (N.S., not significant; two-sided unpaired t test). Scale bar, 5 μm.
