Fig. 3: Glycerol serves as an osmolyte that regulates Imh1.
From: Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1
a, Model of glycerol transport into yeast cells and the glycerol metabolism for G3P production. Glycerol enters yeast cells through the Stl1 channel and is then phosphorylated by Gut1 to produce G3P, which is the substrate of Gpp1/2 (ref. 26). b, Glycerol restores mislocalized Imh1 independent of Gut1. Cells expressing mCH–Imh1 were grown to midlog phase in YPD medium, the medium was removed and the cells were suspended in YPG for 10 min. Scale bar, 5 μm. c, Glycerol specifically rescues the Imh1 localization in glycerol-deficient cells. mCH–Imh1 was expressed in cells. Cells were grown to midlog phase in YPD medium, the medium was removed and the cells were suspended in YP medium with 2% glucose, 3% glycerol, 1% maltose or 6% ethylene glycol or in YPD medium with 0.1 M glycerol, NaCl or sorbitol. Scale bars, 5 μm. d, Glycerol rescues the localization of Imh1 in gpd1∆ and gpp1/2∆ cells within 10 min. Cells expressing mCH–Imh1 were grown to midlog phase in YPD medium, the medium was removed and the cells were suspended in YP medium containing 0.1 M glycerol. mCH–Imh1 was observed after glycerol rescue for 5 and 10 min. Scale bar, 5 μm. b–d, The numbers of mCH–Imh1 puncta were determined with ImageJ Fiji software (N = 3, n = 50). Data are presented as the mean ± s.d. of three independent experiments. Statistical analysis was conducted using a one-way ANOVA with Dunnett’s post hoc multiple-comparison test.
