By leveraging long-read sequencing, single-cell input scNanoHi-C2 systematically delineates chromatin structural reprogramming in embryonic-stage germ cells (EGCs), highlighting the functional importance of three-dimensional (3D) genome structure in mammalian EGC development. This method provides a powerful tool for probing chromatin architecture in individual cells, particularly in rare or precious biological samples.
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References
Wen, L. & Tang, F. Human germline cell development: from the perspective of single-cell sequencing. Mol. Cell 76, 320–328 (2019). A review that presents advances in the reprogramming of germline cells.
Wang, Y. et al. Reprogramming of meiotic chromatin architecture during spermatogenesis. Mol. Cell 73, 547–561 (2019). This paper reports chromatin structures during spermatogenesis in mice and monkeys using low-input bulk Hi-C.
Du, Z. et al. Polycomb group proteins regulate chromatin architecture in mouse oocytes and early embryos. Mol. Cell 77, 825–839 (2020). This paper reports chromatin structure during oocyte maturation in mice using low-input bulk Hi-C.
He, J., Yan, A., Chen, B., Huang, J. & Kee, K. 3D genome remodeling and homologous pairing during meiotic prophase of mouse oogenesis and spermatogenesis. Dev. Cell 58 (2023). This paper reports chromatin structure during the prophase of meiosis in both male and female mice using low-input bulk Hi-C.
Li, W. et al. scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells. Nat. Methods 20, 1493–1505 (2023). This paper reports a single-molecule sequencing platform-based single-cell Hi-C method, scNanoHi-C, which is the previous iteration of scNanoHi-C2.
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This is a summary of: Lu, J. et al. Single-cell long-read Hi-C, scNanoHi-C2, details 3D genome reorganization in embryonic-stage germ cells. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-025-01604-7 (2025).
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scNanoHi-C2 uncovers dynamic reorganization of 3D chromatin structure in mammalian germ cells. Nat Struct Mol Biol 32, 1869–1870 (2025). https://doi.org/10.1038/s41594-025-01603-8
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DOI: https://doi.org/10.1038/s41594-025-01603-8
