Fig. 2: Common and cell-type-specific effects of mRNA translation perturbations in human cells. | Nature Structural & Molecular Biology

Fig. 2: Common and cell-type-specific effects of mRNA translation perturbations in human cells.

From: Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control

Fig. 2

a, Violin plots of gene-level sgRNA log2 fold changes (mean of the top three sgRNAs per gene by magnitude) in each screen separated into functional gene groups (r-proteins, translation factors, mRNA-associated proteins and ribosome-associated proteins). The number of genes with significant (two-sided Mann–Whitney test, P ≤ 0.1) enrichment or depletion in each screen is indicated above each violin. FC, fold change. b, Heatmaps of data in a. Significant (two-sided Mann–Whitney test, P ≤ 0.1) gene-level enrichment or depletion in each screen indicated by color. White, not significant in the respective screen. Data for genes with more than one annotated TSS (S1 and S2) were analyzed separately. Open and closed circles indicate nonessential and essential genes, respectively, in the common essential gene set from DepMap 23Q4 (ref. 14) and genome-wide CRISPRi screens in WTC11 hiPS cells and H1 hES cells35; x indicates the absence of data for alternative TSSs in DepMap. hESC, human embryonic stem cells; PIC, preinitiation complex; tRNA-iMet, initiator tRNA methionine; SKI, superkiller.

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