Extended Data Fig. 10: Mathematical Model of the IST2 linker.
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
a, Scheme illustrating the setup for the mathematical modelling of the IST2 C-terminal region as two entropic springs (SpringN and SpringC). b, Elastic worm-like chain (WLC) potential energy for SpringN (left) and SpringC (right) upon their extension in wild-type (WT, IST2) and mutant constructs. c, Distribution of bound OSH6 along the axis between the endoplasmic reticulum (ER) and the plasma membrane (PM) in constructs of different lengths (100% refers to WT). d, Left, potential energy of the C-terminal region of IST2 during the shuttle of OSH6 between the ER and the PM in IST2 constructs with a relocated OSH6 binding site. Right, distribution of bound OSH6 along the z-axis between ER and PM in constructs with a relocated OSH6 binding site. WT (IIST2) is indicated as dashed line. c, d, The correspondence of energies or distributions to states during transport is indicated (top).
