Fig. 4: Structural properties of the OSH6 interaction with lipid membranes.
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
a, SEC profiles of OSH6 in absence and presence of 2N2 lipid nanodiscs monitored by the fluorescence of the labeled LTP. The comigration with nanodiscs is reflected in the decreased elution volume. b, SEC profile and SDS–PAGE gel (inset; n = 1) of the OSH6–2N2 lipid nanodisc sample used for cryo-EM analysis. c, Ab initio reconstruction of OSH6 (red) bound to the edge of a lipid nanodisc (white) obtained from a cryo-EM dataset. d, Model of OSH6 fitted into the masked and refined cryo-EM density. The lid helix α1 is colored in green. The interacting IST2 peptide (yellow), which was not included in the sample, is displayed for reference. The bound POPS is shown as space-filling model and the relationship between views is indicated.
