Extended Data Fig. 1: Cryo-EM data processing of IST2 in detergent in complex with OSH6.
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
a, Representative micrograph (n = 13,000), 2D classes and data processing workflow for IST2/OSH6 complex. Particles were sorted in 2D into three categories based on size. IST2 and OSH6 particles were then processed separately as indicated. Data was processed using cryoSPARC. b, Local resolution of the final IST2 map computed in cryoSPARC. c, GSFSC curve used for resolution estimation of final maps of IST2. d, GSFSC curve used for resolution estimation of final maps of OSH6. e, Angular viewing direction distribution of particles contributing to the final map of IST2fl. f, Angular viewing direction distribution of particles contributing to the final map of OSH6.
