Table 2 Data collection and refinement statistics of X-ray structures of OSH6 complexes
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
OSH6ΔN–IST2732−761 (PDB 9HDH) | OSH6ΔN–IST2732−756 (PDB 9HDK) | |
|---|---|---|
Data collection | ||
Space group | C2221 | C2221 |
Cell dimensions | ||
a, b, c (Å) | 61.64, 176.25, 95.71 | 61.37,175.7,96.16 |
α, β, γ (°) | 90, 90, 90 | 90,90,90 |
Resolution (Å) | 32.42–1.84 | 37.08–1.93 |
Rmerge | 19.6 (154.1)* | 8.3 (149.3) |
I/σI | 7.91 (1.38) | 18.0 (1.8) |
Completeness (%) | 97.9 (87.1) | 99.6 (99.4) |
Redundancy | 6.73 (5.19) | 13.7 (14.0) |
Refinement | ||
Resolution (Å) | 1.84 | 1.93 |
No. reflections | 44,425 | 41,459 |
Rwork/Rfree | 17.95/20.97 | 18.51/21.32 |
No. atoms | ||
Protein | 3,485 | 3,422 |
Ligands and ions | 53 | 53 |
Water | 319 | 315 |
B factors | ||
Protein | 31.79 | 43.84 |
Ligands and ions | 32.02 | 39.03 |
Water | 38.13 | 49.21 |
Root-mean-square deviations | ||
Bond lengths (Å) | 0.0067 | 0.0064 |
Bond angles (°) | 0.839 | 0.822 |
Validation | ||
MolProbity score | 1.21 | 1.23 |
Clashscore | 2.98 | 2.59 |
Poor rotamers (%) | 0.26 | 0.27 |
Ramachandran plot | ||
Favored (%) | 97.40 | 96.87 |
Allowed (%) | 2.60 | 3.13 |
Disallowed (%) | 0.00 | 0.00 |
