Fig. 4: Specificity for RNase MRP or RNase P is dictated by the N termini of RPP21 and RMP24.
From: RNase MRP subunit composition and role in 40S ribosome biogenesis
a, Diagram depicting the chimeric constructs used in c. b, A cartoon representation of the solved structure of human RPP21 (PDB ID: 6AHR) and the predicted structure of RMP24. Each of the three shared structural features is depicted in a different color. c, Representative agarose gel stained with ethidium bromide from three biological replicates, showing the results of RIP–RT–PCR experiments. d, Quantitation of the integrated pixel intensity of the RNase MRP RNA signal with the GFP signal background subtracted. The y axis shows the fraction of the intensity of MRP RNA to the sum of intensities for both H1 RNA and MRP species. The quantitation for the RMP24, RPP21 and RPP14 RIP–RT–PCRs is duplicated from Fig. 2g for comparison with the chimeras. The error bars represent the s.e.m. from four replicates for the RMP24, RPP21 and RPP14 experiments, and three replicates for the chimera experiments.
