Fig. 6: NEPRO is an rRNA-processing factor that associates with RNase MRP.
From: RNase MRP subunit composition and role in 40S ribosome biogenesis
a, The abundance of the proteins detected in the indicated IP–MS experiments. The shared components of the RNase P/MRP complexes are highlighted in orange, except RPP14, which is shown in pink. RMP24 is highlighted in purple, NEPRO is highlighted in brown and RPP21 is highlighted in green. b, The genes with the highest Pearson correlation of CRISPR–Cas9-based targeting effect scores to NEPRO in the DepMap database (DepMap Public 24Q2). The shared RNase P/MRP components are shown in orange, except RPP14, shown in pink, and RMP24, shown in purple. c, The abundance of the proteins detected in the indicated IP–MS experiments. The shared components of the RNase P/MRP complexes are highlighted in orange. RPP21 was not immunoprecipitated in the NEPRO IP–MS experiments, and thus is not shown. d, A volcano plot showing the log10(FDR) and fold enrichment from RIP–RNA-seq experiments. The top right inset depicts a representative agarose gel stained with ethidium bromide, showing the results of NEPRO RIP–RT–PCR experiments, performed in triplicate. e, Polysome profiles obtained from the fractionation of cell lysates separated on a 10–50% sucrose gradient. NEPRO-knockout profiles obtained using two different guide RNAs are depicted in brown, and the control profile is depicted in gray. The inset on the top right shows a magnified view of the 40S and 60S traces.
