Fig. 1: Selective ribosome profiling identifies cotranslational clients of the OST-A, GEL, PAT and BOS complexes.
From: Global analysis of translocon remodeling during protein synthesis at the ER
a, Individual complexes and their Flag-tagged subunits (marked by asterisks) characterized by selective ribosome profiling. b, The sample preparation strategy for low-input ribosome profiling. c, OST-A enrichment of transcripts encoding proteins of the indicated type. The enrichment values are calculated as the average of OST48 (n = 2) and RPN2 (n = 1) samples. The number of sequences (N), median and interquartile range are indicated. d, As in c but for MPT. The enrichment values are calculated as the average of TMCO1 (n = 2), CCDC47 (n = 2) and Nicalin (n = 2) samples. e, Topology cartoons for cotranslational clients of the human ER translocon. The total number of proteins encoded in the human genome, the median length of the longest translocated segment (seg.) and the associated accessory factor are indicated for each class.
