Fig. 3: Synchronized recruitment of the GEL, PAT and BOS complexes.
From: Global analysis of translocon remodeling during protein synthesis at the ER
a, Scatter plots of single-pass and multipass membrane protein log2 transcript enrichment by GEL, PAT and BOS, colored by density. b, Interaction profiles for CXCR4. The mean enrichment (line) and range (shaded) for GEL, PAT and BOS (n = 2 replicates each) are shown. c, Scatter plots of TMCO1, CCDC47 and Nicalin peaks for all multipass proteins, calculated as the sum of enrichment values across each peak (length >10 codons) and colored by density. For peak definition, see Methods. d, Metagene plots of MPT (n = 6) recruitment to multipass proteins whose first translocated segment is short (≤50 residues), aligned on the end of the indicated feature. The number of sequences (N), median (line) and interquartile range (shaded) are indicated. e, As in d but for multipass proteins whose first translocated segment is long (≥100 residues). f,g, MPT interaction profiles for DOLK (f) and RFT1 (g). The mean enrichment (line) and range (shaded) for n = 6 replicates are shown.
