Fig. 5: Sec61 channel opening is only required for translocation of long hydrophilic segments. | Nature Structural & Molecular Biology

Fig. 5: Sec61 channel opening is only required for translocation of long hydrophilic segments.

From: Global analysis of translocon remodeling during protein synthesis at the ER

Fig. 5: Sec61 channel opening is only required for translocation of long hydrophilic segments.

a, Topology cartoons and scatter plots for the indicated doxycycline-inducible dual-color reporters expressed by transient transfection in HEK293 cells. Each GFP-tagged substrate is translated in tandem with RFP, separated by a viral P2A sequence that mediates cotranslational ribosome skipping, yielding a GFP-tagged substrate and a free RFP that serves as an internal translation control. Cells were treated during induction with 200 nM ApraA (blue) or dimethylsulfoxide (DMSO) (red) and analyzed by flow cytometry. The length of the longest translocated segment is indicated for each protein. Data are representative of two biological replicates. aa, amino acid; ext, extended. b, A model for nascent chain triage between MPT and Sec61.

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