Extended Data Fig. 3: Structure determination of DNMT3A2-3L-NCP in the combined dataset.
From: Mechanisms of DNMT3A–3L-mediated de novo DNA methylation on chromatin
a, Cryo-EM data processing workflow by CryoSPARC (4.6.0) and RELION 5 of the combined dataset for the DNMT3A2-3L-NCP complex. Half-map cFSC plots were shown on the bottom for the three distinct conformations: a DNA-engaged state (grey, left), an intermediate state (yellow, middle), and an “up” state (cyan, right). The final global nominal resolution was set at 3.10 Å for the engaged state, 3.29 Å for the intermediate state, and 3.16 Å for the “up” state at 0.143 FSC. Particles from each conformation were further processed with focused refinements of DNMT and NCP separately. Boxed 3D classes were selected for further processing. The final global nominal resolution was set at 3.15 Å, 3.28 Å, and 3.15 Å at 0.143 FSC for NCPs from three conformations, and at 3.60 Å, 5.68 Å, and 4.22 Å at 0.143 FSC for DNMT3A2-3L from three conformations. b, Color-coded local resolution maps in two different orientations, related by a 180˚ rotation around a vertical axis for DNMT3A2-3L complexes in three distinct conformations. Half-map cFSC plots and angular distribution plots obtained from CryoSPARC were shown at the bottom for each conformation.
