Extended Data Fig. 9: CryoROLE and RELION5 multi-body analysis of the nucleosome-bound DNMT3A2-3L complex.
From: Mechanisms of DNMT3A–3L-mediated de novo DNA methylation on chromatin
a, Overlays of 3D reconstructions calculated from particles selected along different directions (α, β, and γ) in the conformational landscape. The number of particles used was shown on the left. Relative motion trajectories were shown on the right. The reconstructions were calculated from locations at every 10° from +20° to -20° in α and γ direction, from +20° to -30° in β direction. All reconstructions were calculated from particles within a 5 Å radius around selected coordinates and low-pass filtered by 15 Å. The detailed movements were recorded in Supplementary Video 1. b, Multi-body refinement in RELION5 showed similar motion between the nucleosomes and DNMT3A2-3L, especially the distal part of DNMT3A2-3L. The detailed movements were recorded in Supplementary Video 2.
