Extended Data Fig. 5: Comparison of PtdIns(4,5)P2 binding in the transmembrane regions of various TRP channels.
From: Structural landscape of activation, desensitization and inhibition in the human TRPM4 channel
Only the front subunits with bound PtdIns(4,5)P2 (yellow sticks) are highlighted in color. a Homo sapiens TRPM4 structure in an open state obtained in the presence of Ca2+ and native brain PtdIns(4,5)P2 (this study); b, Mus musculus TRPM3 structure in a closed state obtained in the presence of short-chain PtdIns(4,5)P2 diC8 and chemical agonist PregS38. PregS was not observed in the structure. c, Mus musculus TRPM8 structure in an open state obtained in the presence of Ca2+, short-chain PtdIns(4,5)P2 diC8 and two agonists, cryosim-3 (C3) and allyl isothiocyanate (AITC)40. Ca2+ and the two agonists are not shown here for simplicity. d, Rattus norvegicus TRPV1 structure in a closed state obtained in the presence of long-chain brominated PtdIns(4,5)P2 di(18:1)34. e, Rattus norvegicus TRPV1 structure in a dilated state obtained in the presence of short-chain PtdIns(4,5)P2 diC834. In the same study, a closed TRPV1 structure in complex with PtdIns(4,5)P2 diC8 was also observed (PDB: 8U30), in which the short-chain lipid binds at a lower position similar to where long-chain brominated PtdIns(4,5)P2 di(18:1) binds shown in d. f, Oryctolagus cuniculus TRPV5 structure in an open state obtained in the presence of short-chain PtdIns(4,5)P2 diC835.
