Table 1 Cryo-EM data collection, refinement and validation statistics

Data collection and processing

Magnification

165,000

165,000

165,000

105,000

Voltage (kV)

300

300

300

300

Electron exposure (e⁻ per Ų)

60

60

60

60

Defocus range (μm)

−0.9 to −2.2

−0.9 to −2.2

−0.9 to −2.2

−0.9 to −2.2

Pixel size (Å)

0.738

0.738

0.738

0.775

Symmetry imposed

C₄

C₄

C₄

C₄

Initial particle images (no.)

1,491,638

1,491,638

1,491,638

4,103,287

Final particle images (no.)

32,765

57,360

78,427

153,652

Map resolution (Å)

2.44

3.19

2.78

2.63

 FSC threshold: 0.143

Refinement

Model resolution (Å)

 FSC threshold: 0.143

2.44

3.18

2.78

2.64

Map sharpening B factor (Ų)

−54.8

−89.6

−82.0

−86.3

Model composition

 Nonhydrogen atoms

30,544

27,192

31,232

30,964

 Protein residues

3,804

3,420

3,924

3,876

 Ligands

Ca²⁺: 8, PtdIns(4,5)P₂: 4

Ca²⁺: 4

-

ATP: 4

B factors (Ų)

 Protein

85.41

95.81

92.17

53.54

 Ligand

57.74

43.90

-

64.80

Root-mean-square deviations

 Bond lengths (Å)

0.003

0.003

0.002

0.003

 Bond angles (°)

0.510

0.450

0.435

0.497

Validation

 MolProbity score

1.01

1.30

1.03

1.47

 Clashscore

2.33

4.09

2.45

3.86

 Poor rotamers (%)

0.87

1.39

0.48

1.83

Ramachandran plot

 Favored (%)

98.92

98.59

98.47

97.56

 Allowed (%)

1.08

1.41

1.53

2.44

 Disallowed (%)

0

0

0

0