Extended Data Fig. 6: nelfb and nelfe-FKBP12 homozygous cell line validation.
From: Evolution of promoter-proximal pausing enabled a new layer of transcription control
a) Western blot of whole cells following NELF-E degradation with 500 nM dTAG-13 for 0 to 24 h of treatment. b) Western blot validation of chromatin fraction vs nuclear soluble fractionation. c) Western blot of NELF-B and -E proteins after degradation of either protein for 1 h. Both nuclear-soluble and chromatin-bound proteins were analyzed. d) Quantification of western blot signal in (c) for NELF-E after degradation of NELF-B, and vice-versa (n = 3 biological replicates).
