Extended Data Fig. 7: Effect of NELF-B and NELF-E degradation on Pol II distribution.
From: Evolution of promoter-proximal pausing enabled a new layer of transcription control
a) WashU browser shots at the Nanog gene locus before and after NELF-B and -E degradation. b) Heat maps of spike-in normalized PRO-seq signal. c) Heatmaps of log2 fold changes of normalized PRO-seq signal relative to untreated controls. All heat maps are centered on active TSSs in mESCs. d) Metaprofiles of cluster 3 genes at each dTAG time point, the recovery of pause-like behavior between 30 and 60 min, and the proto-pause observed in S. pombe for reference. The proto-pause region is shaded in the two rightmost panels.
