Fig. 2: Genetic interactions of DNAJC15 with subunits of the TIM23 complex.
From: Stress adaptation of mitochondrial protein import by OMA1-mediated degradation of DNAJC15
a, Cell growth of WT and DNAJC15−/− HeLa cells after siRNA-mediated depletion of DNAJC19 (WT, n = 3; DNAJC15−/− n = 8, biologically independent samples). Data are means ± s.d. b, Cell growth of DNAJC15−/− HeLa cells expressing DNAJC15 (JC15) after siRNA-mediated depletion of DNAJC19 (n = 3, biologically independent samples). Data are means ± s.d. c, Cell growth of DNAJC15−/− HeLa cells expressing non-cleavable DNAJC15 (JC15Δ2) after siRNA-mediated depletion of DNAJC19 (n = 3, biologically independent samples). Data are means ± s.d. d, Cell growth of DNAJC15−/− HeLa cells expressing OMA1-cleaved DNAJC15 (S-JC15) after siRNA-mediated depletion of DNAJC19 (n = 3, biologically independent samples). Data are means ± s.d. e, Quantification of cell growth analysis, determining the area under the curve in a–d. P values were calculated using a two-sided Mann–Whitney U-test. Quantile box plot show median (center line) and the 25th and 75th percentiles, and whiskers show minimum and maximum values (1.5 × IQR distance from median, outliers not shown) (WT, n = 3; DNAJC15−/−, n = 8, DNAJC15−/− + JC15Δ2, n = 3, DNAJC15−/− + S-JC15, n = 3, biologically independent samples). f, Heatmap of growth rates of WT, DNAJC15−/− and DNAJC19−/− HeLa cells after siRNA depletion of the indicated subunits of the TIM23 complex. Data are means (n = 3, biologically independent samples). g,h, Cell growth of WT and DNAJC15−/− HeLa cells after siRNA-mediated depletion of TIMM17A (g) or TIMM17B (h) for 72 h (n = 3, biologically independent samples). Data are mean values ± s.d. i, Quantification of cell growth monitoring the area under the curve in (g,h). P values were calculated using a two-sided unpaired t-test. Quantile box plot show the median (center line) and 25th and 75th percentiles, and whiskers show the minimum and maximum values (1.5 × IQR distance from median, outliers not shown, n = 3, biologically independent samples).
