Fig. 3: The DNAJC15 interactome.
From: Stress adaptation of mitochondrial protein import by OMA1-mediated degradation of DNAJC15
DNAJC15 immunoprecipitation in DNAJC15−/− HeLa cells expressing DNAJC15 and in DNAJC15−/− HeLa cells after chemical crosslinking (DSP, 2 mM) under non-denaturing conditions. a, Volcano plot showing the mitochondrial proteins that interact with DNAJC15, as defined by MitoCarta 3.0. A total of 174 mitochondrial proteins (according to MitoCarta 3.0) exhibited a significant interaction, and these are highlighted in green (permutation-based FDR < 0.05, n = 4, biologically independent samples); those with at least a 1.5-fold enrichment are shown in dark green (FDR < 0.05, log2(FC) < 1.58). P values (y axis) were calculated using an unpaired two-sided t-test. b, Pie chart visualizing the known submitochondrial localization of the 173 significant mitochondrial DNAJC15 interaction partners (according to MitoCarta 3.0) (FDR < 0.05). MOM, mitochondrial outer membrane; IMS, intermembrane space; IMM, inner mitochondrial membrane. c, Schematic of the TIM23 complex subunits interacting with DNAJC15. The blue-white-red color scale indicates fold-enrichment between DNAJC15−/− cells expressing DNAJC15 and DNAJC15−/− HeLa cells (log2(FC)). The asterisk indicates all significantly affected proteins (FDR < 0.05, n = 4, biologically independent samples). d, Network analysis of mitochondrial DNAJC15 interactors with a fold-enrichment higher than 1.58 (FDR < 0.05). The box size is adapted to their fold-enrichment (log2FC) in DNAJC15−/− HeLa cells expressing DNAJC15 compared to DNAJC15−/− HeLa cells. Pathways associated with only one gene name were removed.
