Fig. 1: MitoPerturb-Seq combines single-cell CRISPR screening with whole-cell multiome in heteroplasmic cells. | Nature Structural & Molecular Biology

Fig. 1: MitoPerturb-Seq combines single-cell CRISPR screening with whole-cell multiome in heteroplasmic cells.

From: MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy

Fig. 1: MitoPerturb-Seq combines single-cell CRISPR screening with whole-cell multiome in heteroplasmic cells.

a, Overview of the MitoPerturb-Seq experimental workflow in heteroplasmic MEFs. b, Candidate genes included in the MitoPerturb-Seq pooled gRNA library. c–e, UMAPs showing MitoPerturb-Seq cells, clustered on the basis of overall RNA-seq expression (c), chromatin accessibility (d) and a combined WNN analysis (e), following regression of cell-cycle heterogeneity from the RNA-seq dataset. Cells are colored by cell-cycle phase. f, MitoPerturb-Seq target gene assignments before and after gRNA enrichment. Target genes are colored as in b. The total number of cells and the number of cells with gRNA assignments before and after enrichment are indicated above the plot. g,h, Per-base coverage (g) or percentage of ATAC-seq reads (h) aligning to the mtDNA, following alignment to the standard mm10 genome (light green) or to an NUMT-masked mm10 genome before (dark green) and after (purple) hybridization-capture-based mtDNA enrichment. i,j, Per-cell absolute (i) or fold (j) increase in mtDNA coverage following mtDNA enrichment, compared to initial coverage before enrichment (i) or to cells ordered by initial mtDNA coverage (j). Figure created in BioRender. Van den Ameele, J. (2026) https://BioRender.com/jpd1min.

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