Extended Data Fig. 4: FSEC traces of PfHT1 constructs, rat GLUT5 interaction with D-fructose measured by STD NMR and crystal structure of PfHT1 in complex with 2,5-AHM.
a. FSEC traces of DDM purified sugar-binding site mutants of PfHT1-GFP fusions. b. FSEC traces of DDM purified TM7b mutants of PfHT1-GFP fusions. c. The structure of D-fructofuranose and corresponding STD NMR (red) and 1H NMR (black) spectra in the presence of rat GLUT5-GFP reconstituted into liposomes, with the STD signal producing protons shown on red background. d. (left) binding site of the PfHT1 X-ray complex with 2,5-AHM (pink sticks), and hydrogen bonding residues (sticks), and hydrogen bond interactions shown as dashed lines overlayed with a Podler omit electron density map (contoured at 5σ). (right). Comparison of the two slightly different 2,5-AHM poses observed in the X-ray structure, pairwise represented by chains A and C vs. chains B and D, within the asymmetric unit containing four copies of PfH1, with protein shown as in Fig. 1b .
