Extended Data Fig. 1: FSEC traces of XylE constructs, STD NMR control and competition experiments, and XylE sugar binding or gating helix mutant transport assay.
a. FSEC traces of DDM purified XylE-GFP constructs. b. STD NMR data from protein-free liposomes of different lipid composition in the presence of sugars. c. Competition between D-xylose and D-glucose measured by STD NMR using WT XylE. (top) 1H and STD spectrum with only D-xylose. (bottom) as top but after addition of D-Glucose to the same sample, showing the loss of STD signal after addition of the inhibitory sugar. d. Uptake of 3H-D-xylose or 14C-D-glucose by XylE WT or the mutants Q175I and L297F in proteoliposomes. Error bars indicate mean ± s.e.m. of n 4 or 6 independent experiments.
