Table 2 Cryo-EM data collection, refinement and validation statistics
PfHT1_2,5-AHM | |
|---|---|
(EMDB-54787) | |
(PDB 9SDL) | |
Data collection and processing | |
Magnification | 130,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 62.3 |
Defocus range (μm) | 2.0–0.6 |
Pixel size (Å) | 0.65 |
Symmetry imposed | C2 |
Initial particle images (no.) | 5,741,311 |
Final particle images (no.) | 300,678 |
Map resolution (Å) | 2.42 |
FSC threshold | 0.143 |
Map resolution range (Å) | 2.1–4.2 |
Refinement | |
Initial model used (PDB code) | |
Model resolution (Å) | 2.6 |
FSC threshold | 0.5 |
Model composition | |
Non-hydrogen atoms | 7,604 |
Protein residues | 956 |
Ligands | 2 |
B factors (Å2) | |
Protein | 76.72 |
Ligand | 66.07 |
R.m.s. deviations | |
Bond lengths (Å) | 0.003 |
Bond angles (°) | 0.430 |
Validation | |
MolProbity score | 1.59 |
Clashscore | 2.59 |
Poor rotamers (%) | 0.47 |
Ramachandran plot | |
Favored (%) | 97.48 |
Allowed (%) | 2.52 |
Disallowed (%) | 0.0 |
