Fig. 1: Reconstitution and cryo-EM structure of RAD51–X3C.
From: Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation
a, Top: size-exclusion chromatograms of RAD51–X3C and X3C complexes and molecular weight standards in the presence of ATP/Mg2+ using a Superdex 200 Increase 10/300 column. Bottom: Coomassie-stained SDS–PAGE gels of the indicated fractions are shown, representative of two independent experiments. Protein molecular weight standards: Tg, thyroglobulin; Fe, ferritin; Al, aldolase; Ca, conalbumin; Oa, ovalbumin. b, Cryo-EM map of the RAD51–X3C complex assembled in the presence of AMP-PNP. Individual subunits are labeled and color-coded. Multiple views are shown, related by the indicated rotations. c, Schematic cartoon illustrating the protomer and subunit organization within the octameric RAD51–X3C complex. d, Cartoon representation of the RAD51–X3C complex. Bound AMP-PNP molecules are shown as spheres.
