Extended Data Fig. 5: Cryo-EM data processing flowchart and map quality of RAD51-X3CDX2. | Nature Structural & Molecular Biology

Extended Data Fig. 5: Cryo-EM data processing flowchart and map quality of RAD51-X3CDX2.

From: Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation

Extended Data Fig. 5: Cryo-EM data processing flowchart and map quality of RAD51-X3CDX2.The alternative text for this image may have been generated using AI.

a, Auto-picked particles were first sorted by three rounds of 2D classification, six initial models were made first then followed by four rounds of heterogenous refinement. A final step of stringent heterogenous refinement and tight mask homogeneous refinement led to a final higher resolution map. All data analysis were performed using Cryosparc v4.4.1. b, Final Euler angle distribution of all particles used in refinement. c, Local resolution map, color coded from lower resolution (red) to higher resolution (blue). (c) Detailed views of representative local cryo-EM density from the RAD51-X3CDX2 complex reconstruction. d, Detailed views of representative local cryo-EM density from the RAD51-X3C complex reconstruction. e, Cryo-EM map of the RAD51-X3CDX2complex with two views related by a 180 degree rotation about the y axis. Maps of the individual subunits are shown to the right with RAD51 (light blue), XRCC3 (gold), RAD51C (magenta), RAD51D (dark blue), XRCC2 (salmon).

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