Extended Data Fig. 9: Cryo-EM data processing flowchart and map quality of ssDNA-bound RAD51-X3CDX2.

a, Auto-picked particles (2,424,208) were first sorted by four rounds of 2D classification. Next, three initial models were generated, followed by another two initial models from the largest class. Two further rounds of heterogenous refinement followed by a subsequent homogeneous refinement of a 123,502 particle stack resulted in a map with 3.1 Å resolution. Two final rounds of local refinement yielded a final map with a 2.95 Å nominal resolution. All data processing was performed using CryoSparc v 4.2.0. b, Final Euler angle distribution of all particles used in refinement. c, Local resolution map color coded from lower resolution (red) to higher resolution (blue). d, Shell Correlation (FSC) plot from two half-maps with an overall resolution of 2.95 Å as determined by 0.143 criterion. e, Local EM-densities for bound AMP-PNP, side-chains and ssDNA. f, Cryo-EM maps for the overall XRCC3-RAD51C-RAD51D-XRCC2 paralog and individual subunits.