Extended Data Fig. 9: Viral determinants of DRT1 activation.
From: Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase
a) Phage defense plaque assay of DRT1 with either no tag, a C-terminal 3xFLAG tag, or an N-terminal MBP tag. b) Comparison of total unmapped reads between cDIP-seq samples. c) Dose-response of DRT1wt to dNTP mix titration. Samples run directly on SDS–PAGE (left) or digested with proteinase K and resolved on TBE-urea (right). d) Co-toxicity cell viability assay of DRT1wt with candidate T4 phage trigger genes expressed from an arabinose-inducible promoter (Para). e) LC-MS/MS proteomic analysis, volcano plot of differentially enriched T4 phage proteins following infection of cells expressing DRT1wt versus DRT1RT KO. f) Phylogenetic tree of phage collection used in this study with indicators of sensitivity to DRT1, presence of Dda, Inh, Trna.2 or homologs.
