Extended Data Fig. 1: DRT1 antiphage activity and assay of DNA synthesis by purified DRT1, DRT5S, and DRT5L.
From: Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase
a) Growth curve of cells expressing empty plasmid or DRT1wt +/− T4 phage at the indicated multiplicity of infection (MOI). Data are shown as mean ± s.d. for n = 3 independent biological replicates. b) Burst size of T4 phage infecting cells with or without DRT1wt. Data are shown as mean ± s.d. for n = 2 independent biological replicates per condition. c) Cell survival assay of bacteria following 15 min of T4 phage infection. Each sample shows n = 2 biological replicates, with bars denoting the mean and s.d. d) dNTP polymerization assay of purified DRT1, DRT5S, and DRT5L. Samples were treated with proteinase K, then resolved on 15% TBE-urea gel. A representative gel of n = 2 independent experiments is shown for each sample. e) Covalent attachment of DNA to DRT1 and DRT5 as demonstrated by the necessity of proteinase K digestion to release DNA into the soluble phase during phenol-chloroform extraction. A representative gel of n = 2 independent experiments is shown for each sample. f) dNTP polymerization assay of purified WT (DRT1wt), RT-inactive (DRT1RTKO), and nitrilase-inactive (DRT1NitKO) protein. A representative gel of n = 2 independent experiments is shown.
