Table 1 Cryo-EM data collection, refinement and validation statistics
From: Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase
DRT1 + dNTPs | |
|---|---|
Data collection and processing | |
Magnification | 105,000 |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 55 |
Defocus range (μm) | 0.9–2.4 |
Pixel size (Å) | 0.826 |
Symmetry imposed | D2 |
Initial particle images (no.) | 1,134,805 |
Final particle images (no.) | 181,263 |
Map resolution (Å) | 2.6 |
FSC threshold | 0.143 |
Map resolution range (Å) | 1.79–28.25 |
Refinement | |
Initial model used (PDB code) | AlphaFold 3 model |
Model resolution (Å) | 2.7 |
FSC threshold | 0.5 |
Map sharpening B factor (Å2) | −88.1 |
Model composition | |
Nonhydrogen atoms | 73,480 |
Protein residues | 9,000 |
Ligands | 24 (8 ATP, 16 Mg2+) |
B factors (Å2) | |
Protein | 38.6 |
Ligand | 94.0 |
R.m.s. deviations | |
Bond lengths (Å) | 0.004 |
Bond angles (°) | 1.03 |
Validation | |
MolProbity score | 1.02 |
Clashscore | 1.61 |
Poor rotamers (%) | 0.42 |
Ramachandran plot | |
Favored (%) | 97.41 |
Allowed (%) | 2.59 |
Disallowed (%) | 0 |
