Fig. 1
Experimental design. (a) The datasets generated in these experiments were derived from 21 samples in total (18 limb tissue samples and 3 aquarium water samples). Post amputation limb tissue samples were collected at these experimental timepoints: 0 dpa, 1 dpa, 4 dpa, 7 dpa, 30 dpa and 60 dpa; “dpa” denoting day post amputation. At each experimental time point groups included 3 biological replicates (R1, R2 and R3) and each replicate was formed by pooling limb tissue samples from 3 animals to minimize interindividual variation. The aquarium water samples were collected at 3 time points (day 0, day 30 and day 60) in 3 replicates (R1, R2, and R3). (b) The workflow of data analysis, the employed tools and changes in the number of processed reads at each step are shown.
