Table 1 Primer sequences.
Gene | Primer name | Primer sequence (5′-3′) |
|---|---|---|
KRAS | KRASf | TTATAAGGCCTGCTGAAAATGACTGAA |
5′KRASf clamp | CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCCC | |
KRASr | TGAATTAGCTGTATCGTCAAGGCACT | |
BRAF | BRAF-51F TaqMan | CTACTGTTTTCCTTTACTTACTACACCTCAGA |
BRAF-176R TaqMan | ATCCAGACAACTGTTCAAACTGATG | |
BRAFWT probe | FAM-TAGCTACAGaGAAATC | |
BRAFmut probe | VIC-CTAGCTACAGtGAAATC | |
BRAFFs | TGCTTGCTCTGATAGGAAAATG | |
BRAFRs | CCACAAAATGGATCCAGACA | |
BRAFseq | TGATAGGAAAATGAGATCTAC | |
PIK3CA | PI542-5EF | TGCTTTTTCTGTAAATCATCTGTGA |
PI542-5ER | TGCTGAGATCAGCCAAATTC | |
PI1047EF | CATTTGCTCCAAACTGACCA | |
PI1047ER | GGTCTTTGCCTGCTGAGAGT | |
PI542-5IF | GCTAGAGACAATGAATTAAGGGAAA | |
PI542-5IR | AAGAAAAAGAAACAGAGAATCTCCA | |
PI1047IF | TATTCGACAGCATGCCAATC | |
PI1047IR | TGTGTGGAAGATCCAATCCA | |
PI1047seq | TTTTGATGACATTGCATACA |
