Fig. 4

Multi-assay variation of heterogeneity. (a) Analysis of PDX tumor heterogeneity. Correlation of PDX samples profiles across different scales (rows) and measurement modalities (columns). For every distance scale (e.g. model) we report the average pairwise correlation across all pairs of samples at that scale (e.g. any two samples from the same model). (b) Pathway transcriptional expression heterogeneity within and across tumor models. Grayscale: upregulation of a hallmark pathway (column) in a sample (rows). Rows and columns were sorted using unsupervised hierarchical clustering. Red squares: intra-tumoral heterogeneity for case of c-Myc target pathways. (c) Left: sector-averaged β-catenin and pS6 levels based on IF expression. Right: tumor images with IF staining from 3 sectors in PDX-L2. Purple/green color bars indicate PDX model.