Table 2 List of IF Antibodies used in immunofluorescence assays. All antibodies have been tested and verified for human (Hm) and mouse (Ms) reactivity. Marker set 4 (pVEGFR, pAKT) was not used in analysis due to diffuse staining pattern.
Marker set | Biomarkers | Pathway processes | Prod# | Lot | Vendor | Species Reactivity |
|---|---|---|---|---|---|---|
1 | β-catenin | Wnt signaling | 2849 | 8 | Cell Signaling Technology | Hm, Ms* |
pS6 | Proliferation | 2211 | 23 | Cell Signaling Technology | Hm, Ms | |
2 | pERK | MAPK signaling | 4370 | 17 | Cell Signaling Technology | Hm, Ms |
Ki67 | Proliferation | 11882 | 5 | Cell Signaling Technology | Hm, Ms | |
3 | pStat3 | JAK/Stat signaling | 9145 | 31 | Cell Signaling Technology | Hm, Ms |
E-Cadherin | EMT | 3199 | 15 | Cell Signaling Technology | Hm, Ms | |
4 | pVEGFR | Angiogenesis | 2478 | 15 | Cell Signaling Technology | Hm, Ms |
pAKT | AKT/mTor Signaling | 43506 | 1 | Cell Signaling Technology | Hm, Ms | |
1/2/3/4 | Vimentin | Stromal marker | 9856 | 11 | Cell Signaling Technology | Hm, Ms |
