Fig. 2 | Scientific Data

Fig. 2

From: De novo transcriptome assembly of cold stressed clones of the hexaploid Sequoia sempervirens (D. Don) Endl.

Fig. 2

Overview of the experiment and analysis. 17 coast redwood clones were tested at three temperature levels. RNA was isolated in sufficient quality and quantity for sequencing from 93 samples representing 11 different clones and equimolarily pooled. In total, ~370.7 M paired-end reads were generated. De novo assembly was done with Trinity and CLC Genomic Workbench software using ~95.9 M paired-end quality trimmed and filtered by minimum size of 180 bp reads, respectively. The Trinity de novo assembly based on the filtered reads was annotated using Blast2GO Pro.

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