Table 4 Prior and post-filtering transcriptome summary statistics for potato cultivar-specific coding sequences generated by TransRate.

From: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato

TransRate metrics

Désirée

PW363

Rywal

Pre-filter (initial)

Post-filter

Pre-filter (initial)

Post-filter

Pre-filter (initial)

Post-filter

CONTIG METRICS

No. sequences

350,271

197,839

273,216

159,278

134,755

79,095

Sequence mean length

504

792

516

775

459

707

No. sequences under 200 nt

125,465

25,330

88,230

17,370

52,653

13,198

No. sequences over 1000 nt

57,679

55,837

44,508

42,571

19,175

18,748

No. sequences over 10000 nt

23

23

3

3

1

1

’n90

369

444

366

429

351

390

’n50

1,194

1,209

1,110

1,131

1,227

1,218

GC %

41%

42%

42%

42%

42%

42%

Ambiguous nucleotide (N) %

0%

0%

0%

0%

0%

0%

COMPARATIVE METRICS

No. seq. with CRBB hits*

160,295

138,131

138,443

116,834

66,258

55,239

No. reference seq. with CRBB hits*

29,858

27,642

25,739

23,839

23,549

22,163

coverage50#*

25,991

24,586

21,875

20,620

20,258

19,538

coverage95#*

19,329

18,246

15,664

14,727

14,967

14,470

Reference coverage*

65%

63%

56%

54%

53%

52%

  1. ’The largest contig size at which at least 90% or 50% of bases are contained in contigs at least this length.
  2. *Reference-based summary statistics (merged Phureja DM coding sequences were used as reference).
  3. #Proportion of reference proteins with at least N% of their bases covered by a Conditional Reciprocal Best Blast (CRBB) hit.