Table 7 Mapping of independent dataset to newly assembled cultivar specific reference transcriptome.

From: Cultivar-specific transcriptome and pan-transcriptome reconstruction of tetraploid potato

Reference

Désirée

ITAG/PGSC

PGSC

Désirée

ITAG/PGSC

PGSC

Mapping statistics/Sample

SRR10416847

SRR10416848

Number of input reads

14,953,659

14,610,172

Average input read length

252

252

UNIQUE READS:

Uniquely mapped reads# %

74%

69%

71%

73%

67%

69%

Average mapped length

246

244

246

246

244

246

Number of splices: Total

123,490

104,658

159,842

127,414

110,423

165,595

Number of splices: Non-canonical

40,960

43,764

45,750

40,898

45,459

42,978

Mismatch rate per base %

0.75%

0.94%

1.00%

0.74%

0.93%

1.00%

Deletion rate per base

0.05%

0.04%

0.06%

0.05%

0.04%

0.06%

Deletion average length

3.42

3.46

2.88

3.41

3.47

2.89

Insertion rate per base

0.03%

0.02%

0.04%

0.03%

0.02%

0.04%

Insertion average length

2.21

2.99

2.52

2.24

3.00

2.53

MULTI-MAPPING READS:

% of reads mapped to multiple loci#

4.94%

1.95%

2.74%

4.86%

1.68%

2.52%

% of reads mapped to too many loci

0%

0%

0%

0%

0%

0%

UNMAPPED READS:

% of reads unmapped: too many mismatches

0%

0%

0%

0%

0%

0%

% of reads unmapped: too short

21%

29%

27%

22%

31%

29%

% of reads unmapped: other

0%

0%

0%

0%

0%

0%

Mapping statistics/Sample

SRR10416849

all samples

Number of input reads

14,755,430

44,319,261

Average input read length

252

252

UNIQUE READS:

Uniquely mapped reads# %

49%

44%

46%

66%

60%

62%

Average mapped length

245

243

246

246

244

246

Number of splices: Total

95,409

77,103

115,083

346,313

292,184

440,520

Number of splices: Non-canonical

33,269

31,224

31,065

115,127

120,447

119,793

Mismatch rate per base %

0.75%

0.94%

1.01%

0.75%

0.94%

1.00%

Deletion rate per base

0.06%

0.05%

0.07%

0.05%

0.05%

0.06%

Deletion average length

3.23

3.25

2.83

3.36

3.41

2.87

Insertion rate per base

0.03%

0.03%

0.04%

0.03%

0.02%

0.04%

Insertion average length

2.27

3.15

2.56

2.24

3.04

2.54

MULTI-MAPPING READS:

% of reads mapped to multiple loci#

3.60%

1.19%

1.94%

4.47%

1.61%

2.40%

% of reads mapped to too many loci

0%

0%

0%

0%

0%

0%

UNMAPPED READS:

% of reads unmapped: too many mismatches

0%

0%

0%

0%

0%

0%

% of reads unmapped: too short

47%

55%

52%

30%

38%

36%

% of reads unmapped: other

0%

0%

0%

0%

0%

0%

  1. Mapping statistics for Désirée leaf samples under drought stress to Désirée, ITAG/PGSC merged and PGSC representative transcriptome sequences is shown.
  2. RNA-seq data from Désirée leaf samples under drought stress retrieved from the GEO Series GSE140083 – “Transcriptome profiles of contrasting potato (Solanum tuberosum L.) genotypes under water stress”. No chimeric reads detected.
  3. #Relevant % of mapped reads: % of uniquely mapped reads + % of reads mapped to multiple loci.