Fig. 1
Experimental workflow. (a) Cells were thawed, grown, and expanded until 70–80% confluency in a 150 mm dish. (b) For ChIP-Seq, cells were fixed, collected, and frozen (N = 1 biological replicate per cell line). Libraries were prepared, sequenced, and data analyzed. (c) For ATAC-Seq (n = 14 samples with n = 2 biological replicates), cells were incubated in a transposition reaction, DNA was purified, and amplified with limited PCR. Libraries were prepared, sequenced, and analyzed. Diagram was created using Servier Medical ART (https://smart.servier.com/).
