Fig. 6 | Scientific Data

Fig. 6

From: Serial scanning electron microscopy of anti-PKHD1L1 immuno-gold labeled mouse hair cell stereocilia bundles

Fig. 6

Output figures generated by the ‘towersOfGold’ script for Dataset 3 (Cell #344,45,46,47,48,49,50), continued. (a) Perpendicular views (right, front, top) of a single tower, and the gold beads associated with it. (b) Azimuth direction for each individual tower computed using the ‘LinkTable.csv’ table, and displayed as a red vector pointing north from the center of each tower. The ‘north’ direction for each tower is defined as a vector pointing 180° away from its mechanosensitive direction. (c) Azimuth transform for a single tower (top and bottom views); the red line points north before the transform, while the green line points to a common north (for all towers) after the transform. (d) Azimuth transforms computed for the entire bundle. Red, the original azimuth direction; green, the resulting azimuth direction after the transform. (e) Azimuth transform applied to all towers and associated with gold beads. Using the ‘TowerUsage.csv’ table, all towers excluded from further analysis are displayed in gray. (f) All towers included in the analysis and their associated gold beads were registered to a common, template tower for each row, and the convex hull of aggregated towers computed. Based on the identity specified for each tower, towers were divided into four groups: row1 (kinocilium), row 2 (tall row stereocilia), row 3 (middle row stereocilia), and row 4 (short row stereocilia) towers.

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