Fig. 3 | Scientific Data

Fig. 3

From: Three-dimensional structured illumination microscopy data of mitochondria and lysosomes in cardiomyoblasts under normal and galactose-adapted conditions

Fig. 3

A good SIM reconstruction has reconstruction artifacts only of intensities significantly below that of the biological features of interest. The panels show a single plane SIM image with different linear intensity scaling: all values shown from −593 (negative intensities sometimes result from SIM reconstruction), lowest displayed intensity 0, and with the lowest displayed intensity 198. Most reconstruction artifacts and background haze is already removed from the image by discarding the negative intensity values. A few remaining image features not well reconstructed can here be further filtered out by disregarding intensities up to 198, as the well-reconstructed biological features are of significantly higher intensities.

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