Fig. 5
(a) P. aeruginosa upregulates the expression of lactate permease lldP (red point) and other lactate metabolism genes including lactate dehydrogenases (present in the cluster of dark blue points near lldP) in co-culture with C. albicans. (b) Similarly, lactate dehydrogenase lldA (red point) and other lactate metabolism genes (included in dark blue points near lldA) are upregulated in co-culture with S. aureus as well. (c) P. aeruginosa upregulated alcohol dehydrogenase adh in co-culture with C. albicans (d) but not in co-culture with S. aureus. (e) In complex co-culture P. aeruginosa will have to integrate multiple signals such as the positive influence of ethanol and a possible negative influence of lactate that converge to influence phenazine production. After CF-Seq exploratory analysis, our hypothesis is that the presence of ethanol will supersede that of lactate to promote phenazine production.
