Fig. 4 | Scientific Data

Fig. 4

From: Protein interactors of 3-O sulfated heparan sulfates in human MCI and age-matched control cerebrospinal fluid

Fig. 4

Binding profile of ATIII. The raw LFQ values for (a,b) ATIII were plotted for the different replicas of the different AE conditions. ATIII protein is more abundant in the 3S-HS 2 condition either in the (a) MCI or (b) control sample groups. To validate the dataset, the AE experiments were repeated with pooled CSF samples for each sample group. The presence of the ATIII protein in the eluted fraction was assessed via western blot. For both the (c) MCI and (d) control sample groups, ATIII presence was confirmed, but only in AE performed with 3S-HS 2 oligosaccharide. The input fraction corresponding to the pooled CSF samples is presented together with the molecular weight of each band observed.

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