Table 2 Marker panels, number of annotated images and objects stratified by detection method.
From: Cross-platform dataset of multiplex fluorescent cellular object image annotations
Detection (Commercial Platform) | Micron/Pixel Size (magnification) | Number of Panels | Number of Images Annotated | Total Number of Objects in Multichannel Images | Total Number of Objects in Nuclear-only Images |
|---|---|---|---|---|---|
Sequential IF unmixing (Akoya Vectra 3.0) | 0.5 (20X) | 16 | 131 Multichannel 49 Nuclear-only | 63,714 | 22,358 |
Sequential IF narrowband capture (Ultivue InSituPlex + Zeiss Axioscan) | 0.325 (20X) | 5 | 19 Multichannel | 12,425 | 0 |
Cyclical IF narrowband capture (Akoya CODEX) | 0.3774 (20X) | 2 | 10 Multichannel 2 Nuclear-only | 5,919 | 1,358 |