Table 2 Guide to data files deposited in PRIDE Database.

From: Interactome dynamics of RAF1-BRAF kinase monomers and dimers

Raw Data Files:

Construct(s)

Drug

A/C

Biological Replicate 1

Biological Replicate 2

Biological Replicate 3

a) Data used in the publication

pcDNA3.1

+

29AprCR_Nora1.raw

29AprCR_Nora25.raw

29AprCR_Nora49.raw

29AprCR_Nora1_1_130430110103.raw

29AprCR_Nora25_1.raw

29AprCR_Nora49_1.raw

pFKBP

pFRB

+

29AprCR_Nora2.raw

29AprCR_Nora26.raw

29AprCR_Nora50.raw

29AprCR_Nora2_1_130430142009.raw

29AprCR_Nora26_1.raw

29AprCR_Nora50_1.raw

pFKBP

pFRB

SOR

+

29AprCR_Nora3_1.raw

29AprCR_Nora27.raw

29AprCR_Nora51.raw

29AprCR_Nora3_130430160530.raw

29AprCR_Nora27_1.raw

29AprCR_Nora51_1.raw

pFKBP

pFRB

VEM

+

29AprCR_Nora4.raw

29AprCR_Nora28.raw

29AprCR_Nora52.raw

29AprCR_Nora4_1.raw

29AprCR_Nora28_1.raw

29AprCR_Nora52_1.raw

BRAF(wt)

RAF1

29AprCR_Nora5.raw

29AprCR_Nora29.raw

29AprCR_Nora53.raw

29AprCR_Nora5_1.raw

29AprCR_Nora29_1.raw

29AprCR_Nora53_1.raw

BRAF(wt)

RAF1

+

29AprCR_Nora6.raw

29AprCR_Nora30.raw

29AprCR_Nora54.raw

29AprCR_Nora6_1.raw

29AprCR_Nora30_1.raw

29AprCR_Nora54_1.raw

BRAF(wt)

RAF1

SOR

29AprCR_Nora7.raw

29AprCR_Nora31.raw

29AprCR_Nora55.raw

29AprCR_Nora7_1.raw

29AprCR_Nora31_1.raw

29AprCR_Nora55_1.raw

BRAF(wt)

RAF1

SOR

+

29AprCR_Nora8.raw

29AprCR_Nora32.raw

29AprCR_Nora56.raw

29AprCR_Nora8_1.raw

29AprCR_Nora32_1.raw

29AprCR_Nora56_1.raw

BRAF(wt)

RAF1

VEM

29AprCR_Nora9.raw

29AprCR_Nora33.raw

29AprCR_Nora57.raw

29AprCR_Nora9_1.raw

29AprCR_Nora33_1.raw

29AprCR_Nora57_1.raw

BRAF(wt)

RAF1

VEM

+

29AprCR_Nora10.raw

29AprCR_Nora34.raw

29AprCR_Nora58.raw

29AprCR_Nora10_1.raw

29AprCR_Nora34_1.raw

29AprCR_Nora58_1.raw

BRAF(V600E)

RAF1

29AprCR_Nora11.raw

29AprCR_Nora35.raw

29AprCR_Nora59.raw

29AprCR_Nora11_1.raw

29AprCR_Nora35_1.raw

29AprCR_Nora59_1.raw

BRAF(V600E)

RAF1

+

29AprCR_Nora12.raw

29AprCR_Nora36.raw

29AprCR_Nora60.raw

29AprCR_Nora12_1.raw

29AprCR_Nora36_1.raw

29AprCR_Nora60_1.raw

BRAF(V600E)

RAF1

SOR

29AprCR_Nora13.raw

29AprCR_Nora37.raw

29AprCR_Nora61.raw

29AprCR_Nora13_1.raw

29AprCR_Nora37_1.raw

29AprCR_Nora61_1.raw

BRAF(V600E)

RAF1

SOR

+

29AprCR_Nora14.raw

29AprCR_Nora38.raw

29AprCR_Nora62.raw

29AprCR_Nora14_1.raw

29AprCR_Nora38_1.raw

29AprCR_Nora62_1.raw

BRAF(V600E)

RAF1

VEM

29AprCR_Nora15.raw

29AprCR_Nora39.raw

29AprCR_Nora63.raw

29AprCR_Nora15_1.raw

29AprCR_Nora39_1.raw

29AprCR_Nora63_1.raw

BRAF(V600E)

RAF1

VEM

+

29AprCR_Nora16.raw

29AprCR_Nora40.raw

29AprCR_Nora64.raw

29AprCR_Nora16_1.raw

29AprCR_Nora40_1.raw

29AprCR_Nora64_1.raw

b) Data not used in the publication

BRAF(D594G)

RAF1

29AprCR_Nora17.raw

29AprCR_Nora41.raw

29AprCR_Nora65.raw

 

29AprCR_Nora17_1.raw

29AprCR_Nora41_1.raw

29AprCR_Nora65_1.raw

BRAF(D594G)

RAF1

+

29AprCR_Nora18.raw

29AprCR_Nora42.raw

29AprCR_Nora66.raw

 

29AprCR_Nora18_1.raw

29AprCR_Nora42_1.raw

29AprCR_Nora66_1.raw

KSR1

RAF1

29AprCR_Nora19.raw

29AprCR_Nora43.raw

29AprCR_Nora67.raw

 

29AprCR_Nora19_1.raw

29AprCR_Nora43_1.raw

29AprCR_Nora67_1.raw

KSR1

RAF1

+

29AprCR_Nora20.raw

29AprCR_Nora44.raw

29AprCR_Nora68.raw

 

29AprCR_Nora20_1.raw

29AprCR_Nora44_1.raw

29AprCR_Nora68_1.raw

KSR1

RAF1

SOR

29AprCR_Nora21.raw

29AprCR_Nora45.raw

29AprCR_Nora69.raw

 

29AprCR_Nora21_1.raw

29AprCR_Nora45_1.raw

29AprCR_Nora69_1.raw

KSR1

RAF1

SOR

+

29AprCR_Nora22.raw

29AprCR_Nora46.raw

29AprCR_Nora70.raw

 

29AprCR_Nora22_1.raw

29AprCR_Nora46_1.raw

29AprCR_Nora70_1.raw

KSR1

RAF1

VEM

29AprCR_Nora23.raw

29AprCR_Nora47.raw

29AprCR_Nora71.raw

 

29AprCR_Nora23_1.raw

29AprCR_Nora47_1.raw

29AprCR_Nora71_1.raw

KSR1

RAF1

VEM

+

29AprCR_Nora24.raw

29AprCR_Nora48.raw

29AprCR_Nora72.raw

 

29AprCR_Nora24_1.raw

29AprCR_Nora48_1.raw

29AprCR_Nora72_1.raw

c) Processed Data: MaxQuant (Version 1.3.0.5) Output Files

mqpar.xml

summary.txt

experimentalDesignTemplate.txt

peptides.txt

checksum.txt

parameters.txt

proteingroups.txt

modificationSpecificPeptides.txt

  1. (Co-)transfected constructs: pcDNA3.1 (empty vector, Invitrogen), pFKBP (empty vector), B-Raf (wt) – BRAF(wildtype) in pFKBP, B-Raf (V600E) – BRAF(V600E) in pFKBP, B-Raf (D594G) – BRAF(D594G) in pFKBP, KSR1 – KSR1(wildtype) in pKBP, pFRB (empty vector), Raf-1 – RAF1(wildtype) in pFRB. RAF inhibitor drugs: SOR – Sorafenib (30 μM), VEM – Vemurafenib (30 μM), A/C – A/C Heterodimerizer AP21967 (0.5 μM, Agilent). Biological Replicate 1/2/3: Filename (_1 denotes the second technical replicate)